FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15604653
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA147250.010.2622441
AGAGCGT149200.010.0424318
GAGCGTC158350.09.3711639
AAGAGCG181600.08.2110537
TCGGAAG229300.06.5933523
CTAACTC47600.06.443577330-34
ATCGGAA244150.06.3695932
GCTAACT50200.06.3106430-34
GAAGAGC240300.06.2948426
TAACTCG49400.05.975256435-39
TAGATCT53500.05.78625640-44
GGAAGAG310200.05.3614655
TGCTAAC60550.05.25572430-34
GTGCTAA59700.05.18583330-34
ACTCGTG58750.05.097827435-39
CTCGTGT60100.05.00727535-39
Produced by FastQC (version 0.11.5)