FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14543186
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA12600.066.36151
CTAGAAC12700.066.353793
TCTAGAA16600.051.6338352
TAGAACC47050.018.9763534
CTAGGTC9600.013.949576135-139
GCTAGGT10000.013.82358135-139
CTAGCTA10250.013.486726130-134
GCTAGTC10450.013.36616675-79
CTAGTCC11450.012.57609875-79
AGCTAGG11150.012.010398135-139
GAGCTAG11850.011.66525250-54
TAGCTAG10000.011.51965135-139
GCTAGCT12050.011.472112130-134
GGCTAGT11950.011.44740675-79
AGGCTAG12900.010.49275675-79
AGCTAGA13400.010.4233750-54
CGCTAGC13850.010.189092130-134
TCGCTAG13350.010.139248130-134
GCTAGAA14700.09.5995350-54
ACCGGTC118400.08.269828
Produced by FastQC (version 0.11.5)