FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14543186
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC13350.068.491923
CTCTAGA13850.065.346781
TCTAGAA18200.049.4838262
TAGAACC46700.020.0406594
CTAGCTA9750.010.780899130-134
GGCTAGT11950.010.72526775-79
CTAGTCC11800.010.37344475-79
GCTAGTC12100.010.354279575-79
TAGCTAG9050.010.182541135-139
CTAGGTC10400.010.106814135-139
GAGCTAG12600.09.82818550-54
AGGCTAG12950.09.45224975-79
AGCTAGA14250.09.29647750-54
GCTAGCT12600.08.913757130-134
AGCTAGG11500.08.764457135-139
GCTAGAA16200.08.71076950-54
GCTAGGT11500.08.63925135-139
TCGCTAG12250.08.580716130-134
CCGGTCC119500.08.0731299
ACCGGTC121600.07.75608738
Produced by FastQC (version 0.11.5)