FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13518291
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATTCATCTCGTAT192790.14261418103812087TruSeq Adapter, Index 25 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA134900.023.5775959
AGAGCAC135300.023.4547028
GATCGGA193850.017.2812231
GAAGAGC240600.013.9076226
AAGAGCA238700.013.7166527
GGAAGAG259900.013.12404255
TCGGAAG263150.012.8023793
ATCGGAA290050.011.5905352
CGGAAGA353600.09.7277244
AGTCACA80100.08.2291525-29
GTCACAC84400.07.84399725-29
CAGTCAC89750.07.45659525-29
TCACACA89650.07.272242530-34
CACACAG94650.07.146570730-34
GGTACCG49500.07.0465311
CTCGTAT95850.06.802304740-44
GGTATAG69700.06.5682111
CCAGTCA106950.06.418885725-29
CACAGAT110800.06.182834630-34
GGTATGA95850.06.0650891
Produced by FastQC (version 0.11.5)