FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13518291
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTTGATGGTGTAGATCT209190.1547458920657944Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA199050.016.4410131
AGAGCGT201600.016.2492548
GAGCGTC224050.014.5246639
AAGAGCG247700.013.39946657
GAAGAGC250250.013.1766196
GGAAGAG265650.012.6021455
TCGGAAG276050.011.9713733
ATCGGAA292250.011.4355662
CGGAAGA357800.09.5379934
TAGATCT83500.08.173676540-44
AGTGTAC83800.07.663327725-29
GAGTGTA91950.07.234637725-29
GTAGATC96600.07.16957640-44
GTGTACT94550.07.066151625-29
GTAGGGA99250.06.803879715-19
GTGTAGA102700.06.799811440-44
TGTAGGG99900.06.78843615-19
CGTATCA105400.06.680573560-64
AGATCTC108050.06.62324745-49
GGTACCT27155.9044396E-96.4286481
Produced by FastQC (version 0.11.5)