FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13452343
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG36050.013.9898321
GATCTAG7201.3605573E-511.001223
CTAGCCG9252.0841471E-59.3390766
TGAGTGA52250.09.233562
GAGTGAT54350.09.0092793
TCTAGCC12256.593337E-57.6393925
ATCTAGC13502.1881025E-46.932044
TAGTGTG21500.05.960283815-19
ATGGTTG96100.05.3935358
AGTGATG97900.05.2942124
Produced by FastQC (version 0.11.5)