FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15326333
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA68550.024.157149
GCCACTC76300.021.9864488
AGCCACT111900.015.0559967
AAGCCAC116150.014.3807846
CCGTTAA70250.012.7084744
CGTTAAA74000.011.9670135
ACAAAGC163900.010.7183053
TACAAAG180050.09.9970542
GTTAAAT107800.08.8827346
ATACAAA216300.08.800241
CTCACTA41050.08.69976515-19
TCCGTTA110950.08.3061143
AAAGCCA251100.07.1681275
CCCCTAA53200.07.145473100-104
CCTAATG54200.06.562002105-109
GTTCCGT161100.06.5592261
ACACACT56200.06.4821950-54
TACACAC55850.06.471248650-54
CAAAGCC285850.06.3975484
GTCCCCT59900.06.2741137100-104
Produced by FastQC (version 0.11.5)