FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11842127
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT70950.012.60295315-19
CAGTCAC73500.011.14774530-34
CCAGTCA76600.010.94098230-34
TTGGCTC84100.09.91319140-44
ACATTGG82200.09.68693635-39
ACGTCTG96550.09.57442220-24
CACGTCT81950.09.32986520-24
AGCACAC103500.08.94548215-19
CGTCTGA108750.08.69893220-24
TCACATT94250.08.64705435-39
CCCTTAG20151.0913936E-118.5753485
CCTTAGA21655.456968E-128.3138346
TGGCTCA103750.08.31322640-44
AGTCACA57200.08.2316135-39
TCGTATG93750.08.09410450-54
GTCTGAA115900.08.00077820-24
ATTGGCT87600.07.9884640-44
GTCACAT85850.07.91652935-39
CACATTG111050.07.70195635-39
CTCCAGT99800.07.60400130-34
Produced by FastQC (version 0.11.5)