FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12364787
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA7200.033.01
CTAGAAC7700.029.922083
TCTAGAA12700.018.708662
CTAGATC7650.06.4140-144
TAGAACC46650.05.8649524
ACTAGGA18150.05.6330576140-144
CACTAGG16150.05.439009140-144
CTAGGAT17250.05.3426085140-144
CTAGGTC4702.0832985E-45.208511135-139
Produced by FastQC (version 0.11.5)