FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16580624
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA214050.011.3911221
TCGGAAG236750.010.5280263
AGAGCGT244700.010.3850688
ATCGGAA283500.09.0982722
GAGCGTC287000.08.8293639
AAGAGCG299900.08.809647
CGGAAGA350400.07.4628944
GGAAGAG360850.07.3215225
TAGATCT72250.07.0546340-44
GAAGAGC379550.06.9418126
AGTGTAG81350.06.22991525-29
GAGTGTA88200.05.89298925-29
GTAGCTT105050.05.13962230-34
AGAGTGT106350.05.063270625-29
GTAGATC109250.05.03443540-44
Produced by FastQC (version 0.11.5)