FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005188018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16064275
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCGTAGCGTGTAGATCT7825174.871162875386533Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCGTAGCGTGTAGATCTC565340.35192375628529765Illumina Single End PCR Primer 1 (96% over 33bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG197570.12298718740808409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)