FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15902541
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA183200.014.9646989
AGAGCAC195950.014.0277038
GATCGGA230850.012.1622251
TCGGAAG266950.010.7581713
ATCGGAA300750.09.9324662
AAGAGCA296250.09.6670317
CACCCTA63900.09.03254830-34
GAAGAGC321400.08.8212146
CGGAAGA367550.08.1247434
GGAAGAG350600.08.0865335
CCCTAAT78350.07.789213730-34
CCTAATC90500.06.95023730-34
ACCCTAA90950.06.615159530-34
AGTCACC105450.06.237705725-29
CAGTCAC101150.06.17559925-29
CTCGTAT110100.05.56829340-44
TCTCGTA112600.05.444663540-44
CTAATCC115100.05.351558735-39
CCAGTCA117200.05.157947525-29
GTCACCC127600.05.154906325-29
Produced by FastQC (version 0.11.5)