FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14900786
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGTA77550.022.5625883
AGGTATC98150.017.8985885
TATCCTA64750.017.346198
ATCCTAA66300.017.2665589
GCCAGGT107450.016.5600072
CAGGTAT111800.015.7779584
AGCCAGG89050.015.5717251
GGACTAG26300.015.3849715-19
GTATCCT115100.015.0125757
CTAACCA32200.013.8629317
CCTAACC35950.013.4183179
CTAGACG26550.013.23340720-24
GACTAGA32250.013.08229415-19
ACTAGAC29350.012.36342320-24
GGTATCC148850.012.0923416
TCCTAAC41250.011.8687298
CTAACCG35300.09.5861310-14
TAACCAG48400.08.7765918
GACTGTA93150.08.61054540-44
ACTGTAG84200.07.610377340-44
Produced by FastQC (version 0.11.5)