FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13302479
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA13650.029.5384621
AGTGAGT34450.019.8548621
GTGAGTA38550.018.3035032
TAACCCC41700.015.8848929
ATAACCC68550.010.1881838
TCTAGAA42750.09.4315792
CTAGAAC55000.07.72363663
TGAGTAT104550.07.5753233
GAGTATA99700.07.51053144
GTATAAC110200.06.468246
TATAACC121350.05.7552547
TAGAACC74250.05.33333354
AGCATGA142450.05.05440479
Produced by FastQC (version 0.11.5)