FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16732233
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGA21200.014.26365715-19
GGACTAG22600.013.31635315-19
CTAGACG21100.011.80603620-24
ACTAGAC21750.011.32080520-24
CCAGGTA135900.09.32508853
GCCAGGT137850.09.2498632
TATCCTA100350.08.5378878
CCTAACC55000.08.5089129
ATCCTAA98950.08.2221629
CAGGTAT165500.08.1352744
GTATCCT175050.08.0203267
GGTATCC177000.07.8098196
CTAGACT15000.07.487740515-19
AGGTATC175150.07.3579345
CTAACCA53000.07.19978437
CGGACTA49600.06.473969515-19
AACGTGT97650.06.355596555-59
CTAACCG41550.06.342113510-14
ACGTGTA82550.06.297112555-59
TAGATCT84550.05.892707360-64
Produced by FastQC (version 0.11.5)