FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10772229
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGG114450.05.598282140-144
TCGTGTA133600.05.5717893140-144
GTCGTGT132450.05.5223293140-144
GCGTCGT150500.05.424468140-144
GATCGGA210000.05.409639140-144
GTAGGGA113500.05.366054140-144
CGTGTAG130950.05.310705140-144
AGCGTCG163700.05.2861123140-144
TTAGACG50050.05.2357383140-144
GTGTTAG62000.05.2251816140-144
Produced by FastQC (version 0.11.5)