FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13910980
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA131600.08.72672751
AGAGCGT160500.07.446418
AAGAGCG187900.06.7054077
ACTATAG53150.06.6375339
TCGGAAG182000.06.48782063
GAGCGTC178900.06.39883149
ATCGGAA196950.06.29051542
ACACTAT69900.06.0769937
AGTGAGT37001.1823431E-105.89345171
CCCCTTA35702.2737368E-95.64697934
CACTATA76300.05.56725848
GTGAGTA40252.2919266E-105.54765652
GGAAGAG234300.05.5003455
GAAGAGC243700.05.49504576
CGGAAGA248250.05.45249134
TACCCCT39805.5770215E-95.24842172
AACACTA77450.05.1127576
GTACCCC39902.6104317E-85.1007651
Produced by FastQC (version 0.11.5)