FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13949002
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA16000.067.5739751
CTAGAAC17100.064.021433
TCTAGAA21600.051.0181772
TAGAACC51200.022.2204554
CTAGGTC10550.013.103073135-139
CTAGTCC11450.012.95344375-79
CCGGTCC96750.012.3527979
CTAGCTA11700.012.307604130-134
GCTAGGT11400.012.252402135-139
GCTAGTC11850.011.90861275-79
AACCGGT101250.011.5904217
GGCTAGT12100.011.54356275-79
ACCGGTC107750.010.8244518
GAGCTAG13000.010.52253350-54
GCTAGCT13900.010.463234130-134
CGCTAGC14000.010.182783130-134
AGCTAGG13900.010.048734135-139
AGCTAGA14500.09.73191250-54
TCGCTAG14450.09.566712130-134
TAGCTAG12700.08.957314135-139
Produced by FastQC (version 0.11.5)