FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14349129
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA12150.049.2332951
CTAGAAC13350.044.235273
TCTAGAA18400.032.8774032
TAGAACC56450.010.9695894
GCTAGGT7100.010.546282135-139
CTAGTCC8600.09.8790575-79
CTGAGAG43750.09.5544811
CTAGCTA8600.09.544087130-134
AGCTAGG8650.09.32237135-139
CTAGGTC8450.09.202195135-139
CAGTGGT60700.08.7780674
TGAGAGG44350.08.6063372
AGTGGTA64750.08.4506845
GAGCTAG8650.08.32355650-54
GCTAGTC9300.08.20643575-79
CGCTAGC9800.08.08155130-134
TCGCTAG10150.07.802875130-134
GCTAGCT10500.07.4056377130-134
CCGGTCC87900.07.2079519
AGGCTAG10850.07.16680675-79
Produced by FastQC (version 0.11.5)