FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14349129
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC14500.052.1347243
CTCTAGA15050.049.7864841
TCTAGAA21350.034.7547842
TAGAACC59250.014.0953354
GACTCAC24550.011.4453131
CTCACTC38350.08.6357153
ACTCACT32650.08.6051142
CCGGTCC91900.08.1475149
ACCGGTC106100.07.87125258
GCTAGTC7100.07.30121875-79
TCACTCT48900.06.33089834
CTAGCTA8000.06.2995462130-134
CTAGGTC7902.3646862E-115.8324914135-139
AGCTAGG7851.1823431E-105.686215135-139
GCTAGGT7603.0377123E-105.683801135-139
ACTCTGG53300.05.67313776
TAGCTAG8502.910383E-115.5901856135-139
CTAGTCC8401.2551027E-105.48557275-79
AGAACCG175500.05.2919455
TCGCTAG8601.3296813E-95.1903243130-134
Produced by FastQC (version 0.11.5)