FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16179062
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA67000.020.2022387
ACACTAT82700.016.9763978
CACTATA87200.015.8527729
AAACACT115550.011.9009016
ACGTTAA45550.011.0643894
AAAACAC164750.08.6965185
TACGTTA62700.08.3936983
GGCCTAA33050.08.23455275-79
ACAAAAC184600.07.8889223
GTTACGT65100.07.8627661
CAAAACA198400.07.54813154
CGTTAAA74400.07.0642765
GGCCCCT46050.06.4101944110-114
GTTAAAT94550.06.09178976
ATACAAA273700.05.9002611
GACCCCT52200.05.710144120-124
CCTAATC54200.05.6854057110-114
GCCCCTA54500.05.5220046110-114
TACAAAA297600.05.37796552
ACTATAG57150.05.341583710-14
Produced by FastQC (version 0.11.5)