FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17240436
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTTA74150.023.5891139
AGCCACT119100.014.86786657
AAGCCAC130150.014.0480126
GCCACTT131300.013.5407018
GAGCACA163550.010.6947899
ACAAAGC180350.010.3446643
AGAGCAC181300.09.6475588
GTTATGT93850.09.4681921
GGCCTAA38450.09.43707465-69
TACAAAG215900.09.0088192
GATCGGA210850.08.6359051
GTTAAAT102850.08.6084736
TATGTTA116750.08.0825273
ATACAAA262000.07.61711931
TGTTAAA120000.07.4381645
AAAGCCA281700.07.18033655
TCGGAAG266750.07.04804853
CCCCTAA57850.06.644379100-104
ATGTTAA135700.06.6353364
AAGAGCA285150.06.61383257
Produced by FastQC (version 0.11.5)