FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18750772
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGTC68700.015.5551531
TGTGTCA109650.09.3930492
GTATAGA130450.07.801851
GTATACG62900.07.39678431
GTATAGG72050.07.3655051
CATAAGT139850.07.20750477
GTGTCAT144700.06.86639453
TAAGTCT147300.06.79409179
ATAAGTC149600.06.7377658
GTATAAA172950.06.6412551
GTATATT229450.06.6217361
GTATATG170300.06.61653661
GTATAGC102250.06.61200671
GTATACT88100.06.60127351
GTATCCT165200.06.46875861
GTATCCA146200.06.36467551
GTGTCCA83950.06.32143641
GTCATAA176300.06.24801835
GTATAAG148200.06.1316231
GTGTCTA34601.3278623E-106.0930611
Produced by FastQC (version 0.11.5)