FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13646389
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTAT274720.2013133291158562TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA152750.033.8249559
AGAGCAC163750.031.1572388
GATCGGA171150.030.4642031
TCGGAAG184300.028.5133023
AAGAGCA224050.023.1892267
ATCGGAA229900.023.0777152
GAAGAGC241050.021.494426
GGAAGAG258500.020.3496635
CGGAAGA286450.018.4143124
CACACTA19950.014.7888697
AGTCACA85050.011.71004725-29
CAGTCAC97950.010.54986525-29
CACGTCT104050.010.00046610-14
CACACGT104600.09.89284510-14
AGCGAAC112900.09.6244758
ACTCCAG113400.09.48063120-24
CTCGTAT102950.09.47904540-44
CCAGTCA111900.09.47903825-29
TCGTATG109350.09.0946245-49
ACACGTC118050.08.85104710-14
Produced by FastQC (version 0.11.5)