FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005188272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18597876
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA48600.013.3392254
CCCTTAG60550.010.3507725
TAGAGCC67800.09.1383299
TGCCCCT77300.08.2886492
CTTAGAG89250.07.91058977
GCCCCTT79250.07.9025123
CCTTAGA90450.07.48704156
TTAGAGC91800.07.2200998
GGGAGTT107750.06.21775055
TGGGAGT107500.06.1645834
CTGCCCC104100.05.7768351
GTTCGTG118600.05.64932169
GAGTTCG141750.05.03156477
TAGATCG39500.05.030248140-144
Produced by FastQC (version 0.11.5)