FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005188295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79666136
Sequences flagged as poor quality0
Sequence length126
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC30641783.846274155934963TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2936840.36864345975057705TruSeq Adapter, Index 1 (100% over 49bp)
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA1314700.16502620385655456No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840160.10546011670504517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)