FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005188311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50841784
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC789840.15535253444292985No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT672420.13225735745228767No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC560350.11021446454357307No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT548000.10778536016753464No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC532530.10474258731754968TruSeq Adapter, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)