FastQCFastQC Report
Fri 27 May 2022
EGAF00005188315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61151543
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC19299083.1559432604995754TruSeq Adapter, Index 4 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC9019561.474952152883534TruSeq Adapter, Index 4 (100% over 50bp)
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC792710.12963041668466158No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT623110.1018960388293064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)