FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005188358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005188358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62416705
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT7532421.2067955205261156Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2598000.41623472434182485Illumina Single End PCR Primer 1 (100% over 50bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA1331200.21327623750725067No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA1165560.1867384700938635No Hit
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT711670.11401915560906332Illumina Single End PCR Primer 1 (98% over 50bp)
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC675000.10814412583938866No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC633160.1014407921725442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)