FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61099232
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA349250.017.3303991
TCGGAAG367700.016.5621912
CGGAAGA388750.015.8145763
TATGCCG116900.012.9487945-49
CTCGTAT135750.011.107944540-44
CGTATGC136500.010.7495745-49
TCTCGTA129800.010.14264740-44
ATGCCGT152700.09.92248545-49
AGAGCAC727200.08.9320267
GCCGTCT168150.08.77821250-54
TCGTATG149450.08.66354740-44
AGCACAC759200.08.584199
GAGCACA787650.08.2189078
ATCTCGT164950.08.051620540-44
GAAGAGC806700.08.0337475
CGTCTTC198650.07.846483750-54
TGCCGTC166550.07.808920445-49
CCGTCTT208650.07.241099450-54
AAGAGCA1133950.06.01577576
CGTCTGA267150.05.774661515-19
Produced by FastQC (version 0.11.5)