FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005189169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87438025
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA380550.017.3758981
ATCGGAA433700.015.4816212
TCGGAAG456800.014.728093
CGGAAGA493500.013.64754
TATGCCG167700.012.46707645-49
CTCGTAT189050.011.04383940-44
CGTATGC192700.010.78946445-49
TCTCGTA188650.010.42935340-44
ATGCCGT222750.09.5617345-49
GCCGTCT229300.09.0742850-54
ATCTCGT240600.08.29798540-44
CGTGGTA271400.07.800256330-34
CGTCTTC291050.07.49280850-54
CCGTCTT292650.07.45679750-54
AGAGCAC990500.07.2385158
GTCACGT301450.07.123412625-29
AGTCACG305850.07.082562425-29
GAGCACA1078200.06.8514159
GAAGAGC1118050.06.56822446
TCGTATG218350.06.51400745-49
Produced by FastQC (version 0.11.5)