FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005189174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67251314
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTAGCTGTGTAGATCTC1817600.27026981212590134Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG432650.065.720029
GAGCGTC495700.057.346218
AGAGCGT540200.052.7550857
ATCGGAA546750.051.814711
TCGGAAG571350.049.909152
CGGAAGA607600.046.9965823
AAGAGCG626900.045.561266
GAAGAGC1077850.026.9231175
CGCCGTA265000.022.70520655-59
TCGCCGT284200.021.17638855-59
GCCGTAT302000.020.01945955-59
GTCGCCG307350.019.69928655-59
TGGTCGC329550.018.57481450-54
CGTATCA331600.018.25847860-64
GGAAGAG1629250.018.0471364
GTGGTCG346200.017.76524450-54
CCGTATC322200.017.75639355-59
GGTCGCC335200.017.13280350-54
TCTCGGT392200.015.87380545-49
CGTCGTG415100.014.66711810-14
Produced by FastQC (version 0.11.5)