FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005189182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66249683
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTAGGAGTGTGTAGATCTC1944570.2935214044722297Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG467650.065.911239
GAGCGTC534000.057.7768
AGAGCGT579550.053.398367
ATCGGAA604950.051.4226651
TCGGAAG616750.050.4933552
CGGAAGA656700.047.3670853
AAGAGCG660050.046.984726
GAAGAGC1103700.028.5319525
CGCCGTA284350.023.74202255-59
TCGCCGT300200.022.27113755-59
GCCGTAT316650.021.44842355-59
GTCGCCG316950.021.31832755-59
TGGTCGC340050.020.0362350-54
GGAAGAG1653050.019.361434
GTGGTCG354800.019.24005150-54
CGTATCA351550.019.23189560-64
CCGTATC341250.018.09643255-59
GGTCGCC346550.017.84040350-54
TCTCGGT407100.017.1974745-49
CTCGGTG438400.015.99278845-49
Produced by FastQC (version 0.11.5)