FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005189184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56095540
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCAATCTGTGTAGATCTC1895820.33796269721264827Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1080250.026.9791539
GAGCGTC1156550.025.2118198
AGAGCGT1232300.023.7791527
AAGAGCG1344000.021.9205766
CGCCGTA343050.021.46144355-59
TCGGAAG1433000.020.561712
CGGAAGA1435450.020.519023
TCGCCGT358100.020.5149455-59
ATCGGAA1454250.020.265631
GCCGTAT371650.019.92693155-59
GTCGCCG370500.019.84790455-59
TGGTCGC398150.018.7793750-54
CGTATCA391300.018.7593660-64
GTGGTCG413350.018.13089450-54
GAAGAGC1727900.017.2181575
TCTCGGT480000.015.69184545-49
CGGTGGT490850.015.22095750-54
CTCGGTG497150.015.20010745-49
CCGTATC388850.015.156610555-59
GGTCGCC394800.015.03125350-54
Produced by FastQC (version 0.11.5)