FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005189188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62532519
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA64650.09.37284855-59
AGCGTCG288400.08.1916619
TCGCCGT80200.07.86282755-59
GAGCGTC357050.06.6369748
GCCGTAT96050.06.61059555-59
GTCGCCG97050.06.602228655-59
GCGTCGG48800.06.538505145
CGTCGTG265700.06.332017145
AGAGCGT398000.06.15415337
AGATCGG456600.05.654051145
GATCGGA423650.05.6487503145
TGGTCGC120650.05.46676950-54
GCGTCGT266300.05.4191046145
CGTATCA125400.05.282989560-64
TCGGAAG465350.05.2492272
AAGAGCG483000.05.2214296
TCGTGTA287400.05.0969477145
ATCGGAA460450.05.0940851
GTGGTCG131350.05.03247250-54