FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31236984
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6430772.0587038748683293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8800.010.8855631
CGTTGGA50850.09.2439291
GCCGTCT93000.08.76290245
CCGTCTT90250.07.784410545
CGTCTTC79150.07.633443445
ATCGGAA11250.07.513962
ACGGGAT40150.07.47753249
AAACGGG65150.07.0318557
GATCGCG10552.6722773E-86.99213545
AACGGGA53350.06.9613398
GATTGCG11804.5147317E-96.84681845
GAAACGG69300.06.80332476
CGGGATT38500.06.77179810
CCGAGTA42450.06.6552422
GGAAACG71650.06.48730375
CGGAAGA22500.06.43570231
CCCGAGT63800.06.42483851
CCCGAAT24400.06.1171631
CGAGTAG43150.06.0308953
TGCCGTC100700.05.999871345
Produced by FastQC (version 0.11.5)