FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15242272
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7817965.129130355369593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4200.019.593491
ATCGGAA6650.011.3693872
TATGCCG16500.010.75966445
TCGGAAG7000.09.8478963
CGTTGGA26950.09.0780651
ACGGGAT18050.08.1531869
TGCCGTC15900.08.03929545
AAACGGG29950.07.79517137
AACGGGA24700.07.76358038
CGGAAGA9700.07.5666141
GAAACGG31800.07.5514416
CGGGATT16800.07.43257510
GCGTGAG31650.06.74530122
GGAAACG36250.06.56315
CGTATGC18700.06.487805443
CCCCGGT5206.97359E-56.4157481
CGTGAGC32950.06.27604823
GTCACGA17800.06.24659426
TCGTATG19550.06.17867742
CTCGTAT18850.06.139810641
Produced by FastQC (version 0.11.5)