FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36693645
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18085254.928714495384692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC83450.017.13939940
CCGTCTT92000.015.64243539
GCCGTCT93300.015.32887738
TGCCGTC96950.014.72853437
ATGCCGT100100.014.28704436
TATGCCG102450.013.8937635
CGTCTGA107350.013.7149643
ACCCGTG108600.013.55525619
CACCCGT109500.013.4031718
TCACCCG111750.013.23234717
CGTATGC108650.013.1617933
CTCGTAT107600.013.10364331
GATCGGA10900.013.08004951
ACGTCTG113600.013.0795342
CCCGTGA112650.012.98903120
CACGTCT115400.012.9144591
TCTCGTA104550.012.91153430
TCGTATG112000.012.82735232
CCGTGAG115250.012.77336621
CGTGAGA117200.012.59902222
Produced by FastQC (version 0.11.5)