FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19317163
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9910775.130551520427715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA12000.022.3503231
TCGGAAG12700.019.6201362
ATGCCGT29200.018.83755145
TATGCCG26700.014.48937644
CGGAAGA17050.012.9167923
ACACGTC28400.012.0628139
CGTATGC30450.011.61210442
CGTCTGA31650.011.1050412
TCGTATG32000.010.92508841
CTCGTAT32700.010.75109540
TCTCGTA31350.010.57479539
GCACACG34600.010.2870047
ACGTCTG35400.09.80297911
CACGTCT35750.09.769443510
ATCTCGT33900.09.71363438
CACACGT38400.09.2689448
GTATGCC43050.08.61909643
AATCTCG40550.08.23038437
ACGGGAT27250.07.0205878
CGGGATT26850.06.87669949
Produced by FastQC (version 0.11.5)