FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17701270
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN288720816.31073928593824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA33300.037.851461
TCGGAAG32250.034.008192
CGGAAGA31700.028.7001973
TATGCCG69700.016.74142845
TCGGAAA11850.015.0188922
ATGCCGT56050.013.24814345
ATCGGAG6600.012.1900751
GCCGTCT43250.011.69125745
CGGAAAG11700.011.5975113
GAAGAGC65550.011.4023615
ATCGGGA5600.010.3760751
CGGAAGC15400.010.1110943
CCGTCTT39450.010.03881345
ACGGGAT23500.09.9391588
TCGGAAC4153.2068783E-99.6491862
GTATGCC85900.09.58391744
CGGGATT23050.09.5542559
AACGGGA31750.09.5284417
TCGAGAG6800.09.4875782
AAACGGG37350.09.112296
Produced by FastQC (version 0.11.5)