FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26773622
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1498269655.96066157952032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25250.040.4836161
ATCGGAA26550.036.372472
TCGGAAG25050.035.970863
TATGCCG69000.025.46344245
CGGAAGA33800.024.6824724
ATGCCGT46500.019.45786745
CGTAACT5850.017.87112216
GATCAAG39250.016.5113031
ACGTAAC6600.015.50328415
GTATGCC84850.014.79287744
CGTATGC80900.014.23948743
ATCGGAG5350.014.1407592
ATCAAGC33200.014.0743652
TCGTATG81600.013.77548742
CTCGTAT81550.013.57723141
GATCGAA4650.013.4101321
TCTCGTA79300.013.30532540
ACGTCAC7700.013.28852915
CGTCTGA56500.013.22819813
ACACGTA11400.012.87801413
Produced by FastQC (version 0.11.5)