FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12804846
Sequences flagged as poor quality0
Sequence length25-295
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG1608.863026E-792367.96265-269
GGCTATA67450.028484.117275-279
TAAGGGT68150.028191.541280-284
GTCAAGA111650.017207.824285
GGTATGG64001.8180694E-515009.794270-274
AAGGGTC72202.3137953E-513305.08280-284
GTATGGC57004.3262633E-511235.401270-274
TGGTCCA100904.5189016E-59520.583270-274
GCTATAA125556.996551E-57651.3486275-279
CTATAAG88901.0523541E-47203.801275-279
GGGTCAA79201.6704299E-46064.5635280-284
AGGGTCA106501.5102712E-46013.3135280-284
GAACCGA17353.3799876E-44814.569265-269
CGAGAGA30753.2732895E-44806.1377265-269
AACCGAT13603.8169973E-44557.053265-269
TATAAGG113153.409391E-44244.9263275-279
ATAAGGG91353.7036193E-44206.3574275-279
GGTCAAG94103.929952E-44083.4297280-284
TATGGCT138355.0970446E-43471.7268270-274
ATGGCTA119556.3430145E-43214.142270-274
Produced by FastQC (version 0.11.5)