FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21357885
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3943181.846240861396154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC21500.013.3091921
GATCGGA4900.011.8619861
CCGCGCC23550.011.5276092
TATGCCG11250.011.27183445
ACCGCAC18350.011.2080971
CGCGCCC17050.010.440183
GACGCGA2251.756745E-49.9356811
ACGCGAG2801.6272803E-59.5365992
CGCGATC13300.09.3686883
CTCACGC55300.09.0933873
CGCGCCT10150.08.7687223
CGAGGCG17000.08.76867735
GCGCGAT13450.08.7684722
CCGCACC19800.08.6535812
GCTCACG56500.08.6251362
GGATCAC107650.08.60570245
GGCGCGA14150.08.3733571
ACCGTGC26950.08.2121451
CGCCTGT66000.07.95488267
AGGCCGA49050.07.93768331
Produced by FastQC (version 0.11.5)