FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39350317
Sequences flagged as poor quality0
Sequence length35-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19093234.8521159308576856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35850.019.32691645
TACGGCG2157.2702096E-712.3625396
CTCGTAT39450.011.59933541
CGTATGC43000.011.08317743
CGGAAGA35300.011.0608491
TCTCGTA38900.010.97859340
ACACGTC41600.010.32915110
CGTCTGA45150.09.86046613
GATCGGA17700.09.1495571
TCGTATG51300.09.11092442
ATCTCGT50250.08.70792939
GTATGCC62600.08.40019344
ATCGGAA19850.08.3720572
ACGTCTG62700.07.31240412
GCCGTCT30300.07.269860740
TGCCGTC30400.07.233838639
CGTCTTC33100.07.060277542
AAGGTAC71550.06.937227731
CACGTCT64850.06.89920111
ATGCCGT33750.06.639355738
Produced by FastQC (version 0.11.5)