FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31723596
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN351430.11077874021595786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA58250.010.3618061
CGTATAA26700.08.508461
ACGGGAT44000.08.3930629
AACGGGA56650.08.2862068
CCCATAA86800.08.2365891
AAACGGG70200.08.1446547
ACTAGAC109300.08.03973810
TATCCAC141650.07.90010456
GGAAACG74950.07.83634765
GAAACGG76900.07.6087076
CGTATGC12505.456968E-127.593137745
GTGATGT186000.07.519427341
TGTGTGC169850.07.430130545
GATGTGT157000.07.265150543
CGGGATA14200.07.20666710
CCCGTAA13250.07.05985071
ATCCACT159200.06.93130877
ACTTGCA176350.06.900429711
CACTTGC177550.06.82874410
CCAAATA204350.06.73559471
Produced by FastQC (version 0.11.5)