FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25866958
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2334780.9026109680156438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9850.015.72267445
CGTTGGA58900.010.5893321
ACGGGAT39350.010.2326329
AACGGGA53750.09.3537028
AAACGGG64400.09.2922487
CGGGATT39000.09.2406810
GAAACGG68550.08.8270896
CTCGTAT12750.08.73380241
GGAAACG69600.08.6305345
CGTATGC12650.08.51020443
TCTCGTA11800.08.48622440
GTCACGA13450.07.774527526
CGAGTTA13350.07.499279530
CCCGTAA10850.07.39092161
ACCGCGC20700.07.09354931
CGCCCGG28100.07.046736235
TCGTATG14850.06.782335842
ACAGGCG88900.06.78162518
CCGAGTA40050.06.77961352
CACCGCG23050.06.756400630
Produced by FastQC (version 0.11.5)