FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28368707
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2232930.7871102479221207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10650.013.96544745
CGTTGGA67600.012.3230071
AACGGGA70200.010.3614618
AAACGGG83850.010.3460057
ACGGGAT50200.010.1471049
GAAACGG86850.09.8095226
GGAAACG89300.09.4661845
CGGGATT47750.09.36338910
GATCGGA6602.6429916E-97.42455671
GTCACGA13350.07.33214726
CGGGAAT32650.07.28972210
CGAACAG13800.07.25405530
CGTCCGA3150.00517845257.0616846
AACGGGT17600.06.95120958
CGTATGC13950.06.730722443
GTTGGAA143900.06.69600442
CGGGATA10055.456968E-126.639961710
TCTCGTA14150.06.60429840
CTCGTAT13400.06.48080241
CACGAAC15100.06.335067728
Produced by FastQC (version 0.11.5)