FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39995243
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1025880.2565005043224765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT50500.07.08862738
ACCGCGC28600.06.998230
AACGGGA68200.06.74860767
ATCGGAA14800.06.66549541
AAACGGG86800.06.5576536
GGTCGAT6700.00184361086.336126345
GAAACGG91000.06.3040885
CACCGCG31150.06.282552729
CGGGATT49450.06.2499449
GGAAACG92050.05.96803674
GCTCTAT111550.05.930474345
GCGTGAG91950.05.803978421
ACAGGCG127500.05.78960617
CGTGAGC95250.05.649657722
GCCACCG84450.05.556507627
CCGCGCC36450.05.491008831
ATCGCGC12803.2208744E-55.2512345
CGCGCCC28000.05.24160432
GAGCCAC272050.05.190696225
GGCGTGA106200.05.171563620
Produced by FastQC (version 0.11.5)