FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28507535
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2854381.0012721198097274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA70800.010.6920551
AACGGGA66850.09.4167348
ACGGGAT49700.08.9960179
AAACGGG81900.08.8541927
CGGGATT46550.08.69693510
GAAACGG85700.08.3329336
GGAAACG86650.08.3190415
TATGCCG19800.08.21271345
CGGGAAT33400.07.059499310
GATCGCG9602.432098E-76.99642845
ACCGCGC25000.06.58492531
GATTTCG35150.06.32953075
ACGGGAA32050.06.31573639
CACCGCG27750.06.25282330
CGCCCGG37250.06.0918535
ATTTCGT41350.06.0258216
CCGCGCC27750.06.01269232
CGCGCCC21750.05.932543833
CCCAAAG293050.05.88810441
GTTGGAA149050.05.88187362
Produced by FastQC (version 0.11.5)