FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005189234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005189234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18903664
Sequences flagged as poor quality0
Sequence length35-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4736672.5056888442367575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC21400.016.2348651
GCGCGAT12050.016.062222
CGCGATC12700.015.765493
GGCGCGA13250.015.1274061
CCGCGCC24000.014.9240962
CGCGCCC17250.013.9284683
GATCGGA4650.012.4525491
ACCGTGC19650.011.4471321
ACCGCAC16550.011.3036621
CGCGCCT10600.011.1234283
GGCGCAA10950.010.5761381
GCGATCT26600.010.1198124
CGCCCGG26150.09.9527695
GCGCCCG21600.09.8874924
CCGCACC17000.09.42232
CCGTGCC21800.09.3886842
GCGCAAT10900.09.3886842
GCGTGAT16050.09.148342
GCCGTCT37850.09.14013545
CGCAATC10600.09.0246693
Produced by FastQC (version 0.11.5)